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d nm 003019 rc220332l3v rc217867l3v rc207605l3v rc203246l3v rc204746l3v origene  (OriGene)


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    Structured Review

    OriGene d nm 003019 rc220332l3v rc217867l3v rc207605l3v rc203246l3v rc204746l3v origene
    D Nm 003019 Rc220332l3v Rc217867l3v Rc207605l3v Rc203246l3v Rc204746l3v Origene, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d+nm+003019/SFTPA1+(NM_005411)+Human+Tagged+ORF+Clone+Lentiviral+Particle/10__1164_slash_rccm__202201___0011OC_ascii32__ascii32_-315-36-43
    Average 90 stars, based on 1 article reviews
    d nm 003019 rc220332l3v rc217867l3v rc207605l3v rc203246l3v rc204746l3v origene - by Bioz Stars, 2026-09
    90/100 stars

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    Transduction:

    Article Title: Pulmonary Surfactant Proteins are Inhibited by IgA Autoantibodies in Severe COVID-19
    Article Snippet: .. HEK293 cells (purchased from CLS Cell Lines Service, Cat. No. 300192) or A549 cells (ATCC, Cat. No. CRM-CCL185) were transduced with lentiviral particles encoding the surfactant-associated proteins A1 (NM_005411), A2 (NM_205854), B (NM_000542), C (NM_003018) and D (NM_003019) (RC220332L3V, RC217867L3V, RC207605L3V, RC203246L3V, RC204746L3V, Origene) and selected with 0.5 μg/mL puromycin (Invivogen) for three passages. .. The cells were further expanded, and HEK293 cells were used for affinity purification of over-expressed proteins via the encoded Flag tag (Anti-FLAG M2 Affinity gel, Merck) using a chromatography system (Äkta pure 25 L1, Cytiva) or lysed and immunoblotted for the detection of Flag-tagged surfactantassociated proteins using the monoclonal anti-Flag BioM2 antibody (Merck) at a dilution of 1:2,000.



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    OriGene d nm 003019 rc220332l3v rc217867l3v rc207605l3v rc203246l3v rc204746l3v origene
    D Nm 003019 Rc220332l3v Rc217867l3v Rc207605l3v Rc203246l3v Rc204746l3v Origene, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d+nm+003019/SFTPA1+(NM_005411)+Human+Tagged+ORF+Clone+Lentiviral+Particle/10__1164_slash_rccm__202201___0011OC_ascii32__ascii32_-315-36-43
    Average 90 stars, based on 1 article reviews
    d nm 003019 rc220332l3v rc217867l3v rc207605l3v rc203246l3v rc204746l3v origene - by Bioz Stars, 2026-09
    90/100 stars
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